FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19050
Sequences flagged as poor quality0
Sequence length48-31638
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAAT9745.1128608923884515No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAAT3932.062992125984252No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC2311.2125984251968505No Hit
CTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC1740.9133858267716535No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCC1030.5406824146981628No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGAATC750.39370078740157477No Hit
CTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAATC740.3884514435695538No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTGAGAACTTGGCC630.3307086614173228No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAAGGAA580.30446194225721784No Hit
GCCATGACCATTATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC480.25196850393700787No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGAACTTGGCC450.23622047244094488No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCCTCAGGAA420.2204724409448819No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGAACTTGGCCA420.2204724409448819No Hit
CCCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAA410.21522309711286086No Hit
GCCATGACCATTGTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCACC380.1994750656167979No Hit
CCTGAAGAAATGTCACGGCATTGGCGCAGGCCGCCTGCTCTCAGGAATCT380.1994750656167979No Hit
CCTGAAGAAATGTCACGGCTTATTGGCGCAGGCCGCCTGCTCTCAGGAAT360.1889763779527559No Hit
CCTGAAGAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC350.1837270341207349No Hit
CCTGAAGAAATGTCGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT330.1732283464566929No Hit
GCCATGACCATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCAC330.1732283464566929No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGAATC270.14173228346456693No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGGGAACTTGG270.14173228346456693No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTGAGAACTTGGCCA260.13648293963254593No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCAGGAATCT230.12073490813648292No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTAGAACTTGGCCAC230.12073490813648292No Hit
GCCATGACCATTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCCACC230.12073490813648292No Hit
CCTGAAGAAATGTCACGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC220.11548556430446195No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAATTGTAGGGAACTTGGCC220.11548556430446195No Hit
CTGAAGAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT210.11023622047244094No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCACCTGCTCTCAGGAAT200.10498687664041995No Hit
TGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT200.10498687664041995No Hit
GCCATGACCATTGTTATTTGTCCAGGGTCAAAATTGTAGGGAACTTGGCC200.10498687664041995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)