FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258573674
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG904050.065.649179
GAGCGTC1120900.053.147428
AGAGCGT1323000.045.2092747
TCGGAAG1234550.044.4024542
ATCGGAA1047150.043.3229371
CGGAAGA1450050.041.3714373
AAGAGCG1571550.038.2479486
GAAGAGC3121550.019.7546565
CGCCGTA621500.018.90778755-59
TCGCCGT722900.016.2476455-59
GCCGTAT763100.015.59207655-59
GTCGCCG803600.014.81703355-59
CCGTATC827350.014.2051555-59
CAGTACG865800.013.84625230-34
CGTATCA868700.013.76455560-64
GCGTCGT898700.013.44529710-14
TGGTCGC899250.013.3866150-54
CGTCGTG927250.013.07660910-14
GGTCGCC926500.012.844974550-54
AGTACGT953400.012.83436635-39
Produced by FastQC (version 0.11.5)