FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437909435
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG14436090.3296592593397765TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2874950.084.034231
TCGGAAG3014050.080.096772
CGGAAGA3223100.074.8306663
AGAGCAC5664250.042.8540087
AGCACAC5967900.040.6506469
GAGCACA6056850.040.0930068
GAAGAGC6206200.039.2368055
AAGAGCA8671750.028.3518316
GGAAGAG9637200.025.617414
TATGCCG2096400.023.34976645-49
CGGCTAT2179900.022.43457430-34
CTCGTAT2122650.021.94827340-44
CGTATGC2222700.021.73989345-49
TCTCGTA2167900.021.47410240-44
ATGCCGT2376800.020.60608545-49
ACCGGCT2380850.020.59645730-34
TGCCGTC2363350.020.38045145-49
GCCGTCT2399000.020.08358450-54
TCGTATG2293600.020.00203140-44
TCACCGG2423000.019.93213525-29
Produced by FastQC (version 0.11.5)