FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443490622
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG14520220.32740759961323374TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2719950.087.242081
TCGGAAG2874450.082.585822
CGGAAGA3091800.076.7639543
AGAGCAC5568100.042.9618647
AGCACAC5860000.040.7426769
GAGCACA5971200.040.110188
GAAGAGC6087050.039.373585
AAGAGCA8552250.028.2974266
GGAAGAG9631900.025.265784
TATGCCG2085400.023.08379445-49
CTCGTAT2114000.021.76866140-44
CGTATGC2203850.021.66350445-49
CGATAGA2225200.021.34036435-39
TCTCGTA2158400.021.28929140-44
AGCGATA2259900.021.27396230-34
GCGATAG2229950.021.23265530-34
ATGCCGT2378800.020.29516445-49
TCGTATG2275900.019.95702240-44
TGCCGTC2387400.019.90321245-49
GCCGTCT2425700.019.60882450-54
Produced by FastQC (version 0.11.5)