FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475885766
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG11772640.24738373872691122TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2304800.081.877351
TCGGAAG2495100.075.9239962
CGGAAGA2727700.069.401823
AGAGCAC5432850.035.280227
AGCACAC5761700.033.2453239
GAGCACA5902500.032.5245368
GAAGAGC6014450.031.981945
AAGAGCA8630050.022.5693876
TATGCCG1781650.021.9257845-49
CTCGTAT1837200.020.51236740-44
CGTATGC1917200.020.13661645-49
GGAAGAG9787150.020.0450744
TCTCGTA1885300.019.95827140-44
ATGCCGT2075200.018.91855645-49
TCGTATG2025250.018.1646140-44
TGCCGTC2116750.018.00542445-49
GCCGTCT2157400.017.9065550-54
ATCTCGT2129750.017.7879840-44
ACTCCGG2295750.017.12958130-34
CCGGAGA2252100.017.01994330-34
Produced by FastQC (version 0.11.5)