FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475885766
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC12390710.26037151949613047Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2921850.064.9265759
GAGCGTC3308600.057.4509168
ATCGGAA3485400.055.0163761
AGAGCGT3652550.052.140197
TCGGAAG3697500.051.953222
CGGAAGA3859200.049.765883
AAGAGCG4154350.046.2122546
GAAGAGC7161900.027.2727345
CGCCGTA2041200.018.8299455-59
GGAAGAG10997100.018.0883644
TCGCCGT2231950.017.2343155-59
GCCGTAT2286750.016.9626855-59
GTCGCCG2444450.015.88437455-59
CCGTATC2399500.015.76142255-59
CGTATCA2466900.015.68159360-64
TGGTCGC2556700.015.33257250-54
GGTCGCC2632050.014.54112250-54
GTGGTCG2720600.014.46749250-54
GCGTCGT2840750.013.68424610-14
CGTCGTG2905350.013.40693410-14
Produced by FastQC (version 0.11.5)