FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486335836
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC6957470.14305896224353082Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2083700.051.0967949
GAGCGTC2484050.042.9872068
ATCGGAA2734600.039.484751
AGAGCGT2810950.038.0731127
TCGGAAG2889400.037.320162
CGGAAGA3079950.034.9849973
AAGAGCG3234550.033.288746
GAAGAGC6428650.017.3259835
CGCCGTA1320650.016.35420455-59
TCGCCGT1465650.014.74020455-59
GCCGTAT1587750.013.72265655-59
GTCGCCG1621250.013.39617155-59
CCGTATC1708100.012.574155-59
TGGTCGC1763150.012.5282550-54
CGTATCA1783800.012.25188960-64
GGTCGCC1807050.011.97352450-54
GTGGTCG1884500.011.80153550-54
CAGTACG1879650.011.4698430-34
GGAAGAG10414500.011.0452354
AGTACGT2010150.010.928349535-39
Produced by FastQC (version 0.11.5)