FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453177317
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG6106160.13474107751955292TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1618500.060.2284431
TCGGAAG1783400.054.6589052
CGGAAGA1986600.049.1849753
AGAGCAC4577650.021.8150227
AGCACAC4907500.020.3671979
GAGCACA5032600.019.9286048
GAAGAGC5130150.019.7520565
TATGCCG1079350.018.53787245-49
CTCGTAT1168150.016.59801540-44
CGTATGC1212650.016.45944245-49
TCTCGTA1215500.015.94069940-44
GTCACGA1350000.014.92308125-29
ATGCCGT1383450.014.64961645-49
TCGTATG1330050.014.311625540-44
TGCCGTC1401150.014.15195445-49
GCCGTCT1440600.013.927340550-54
AAGAGCA7644550.013.5334016
ACGAGAT1503900.013.49027930-34
ATCTCGT1466500.013.3636340-44
AGTCACG1591950.012.77431325-29
Produced by FastQC (version 0.11.5)