FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453177317
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC6418210.14162690318412383Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1942050.050.1296929
GAGCGTC2316350.042.1230968
ATCGGAA2511100.039.464721
AGAGCGT2637700.037.202857
TCGGAAG2670000.037.118232
CGGAAGA2858700.034.6935543
AAGAGCG3084550.032.085976
GAAGAGC6026250.016.9607165
CGCCGTA1227500.015.942656555-59
TCGCCGT1382650.014.15369755-59
GCCGTAT1477700.013.46110255-59
GTCGCCG1536200.012.89846955-59
CCGTATC1581950.012.3604855-59
CGTATCA1651950.012.05540560-64
TGGTCGC1664050.012.0126950-54
GGTCGCC1711650.011.55580650-54
GTGGTCG1782850.011.25125850-54
GGAAGAG9661600.010.9653084
GCGTCGT1878950.010.551578510-14
CGTCGTG1931950.010.283874510-14
Produced by FastQC (version 0.11.5)