FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443412543
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG20483440.4619499453356691TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3520300.095.230111
TCGGAAG3686900.090.994532
CGGAAGA3871000.086.620793
AGAGCAC6400450.052.6009757
AGCACAC6674250.050.3234259
GAGCACA6806400.049.5145538
GAAGAGC6901850.048.9540185
AAGAGCA9366800.036.305746
GGAAGAG10428850.032.7640654
TATGCCG2773650.024.549545-49
CTCGTAT2809750.023.54754440-44
CGTATGC2904050.023.15915545-49
TCTCGTA2873500.023.11036340-44
CGCTCAT3037750.022.390830-34
ATGCCGT3032550.022.3604145-49
TATCTCG2934600.022.34079740-44
TCGTATG2948000.021.9546240-44
ACCGCTC3121950.021.77902430-34
TGCCGTC3077300.021.52887545-49
GCCGTCT3132050.021.39215950-54
Produced by FastQC (version 0.11.5)