FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443412543
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC21146330.47689968030516444Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3847950.087.4211969
GAGCGTC4228800.079.618238
ATCGGAA4448000.076.3908161
AGAGCGT4568850.073.840197
TCGGAAG4632200.073.2988052
CGGAAGA4788150.070.8583763
AAGAGCG5043750.067.37346
GAAGAGC7870300.043.5930825
GGAAGAG11444050.030.3241024
CGCCGTA2909650.023.09011855-59
TCGCCGT3072500.021.79407955-59
GCCGTAT3153900.021.4366455-59
GTCGCCG3289850.020.63629755-59
CCGTATC3258700.020.30404555-59
CGTATCA3332350.020.22839460-64
TGGTCGC3405800.019.97190950-54
GGTCGCC3465450.019.31598950-54
GTGGTCG3560800.019.12127350-54
GCGTCGT3801300.018.04206510-14
CGTCGTG3839550.017.84949110-14
Produced by FastQC (version 0.11.5)