FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189912271
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA649350.046.9204141
TCGGAAG749000.044.9478652
CGGAAGA844400.039.8265463
CGCGAAA336550.020.12329535-39
TCCGCGA347100.019.71736330-34
CCGCGAA362850.018.47812530-34
AGAGCAC1896400.018.3787527
AGCACAC2018250.017.2542939
GAGCACA2075900.016.834458
TATGCCG410950.016.83368545-49
GAAGAGC2125050.016.5651055
CGTATGC467000.014.7357145-49
CTCCGCG482150.014.40491830-34
GCGAAAT480200.014.36600535-39
CTCGTAT418750.014.14634740-44
TCTCGTA437350.013.42875540-44
ACTCCGC527150.013.27695430-34
ATGCCGT540400.012.87902645-49
TGCCGTC543100.012.50816445-49
GCCGTCT551900.012.32695150-54
Produced by FastQC (version 0.11.5)