FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300319907
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA988300.054.467621
TCGGAAG1214550.052.545852
CGGAAGA1354800.047.0953753
AGAGCAC3036100.021.4250227
AGCACAC3269050.020.1020269
GAGCACA3316000.019.7824678
GAAGAGC3397900.019.4191635
TATGCCG708300.018.22861545-49
CGTATGC794800.016.23382245-49
CGATAGA795150.016.06676735-39
AGCGATA819150.015.81913330-34
GCGATAG813550.015.64505630-34
CTCGTAT724200.015.48945440-44
TCTCGTA757850.014.68897940-44
ATGCCGT903950.014.345695545-49
TGCCGTC897250.014.17366945-49
GCCGTCT924000.013.76800550-54
CAGCGAT975400.013.41122630-34
AAGAGCA5067650.013.2017226
TCGTATG842050.013.12732240-44
Produced by FastQC (version 0.11.5)