FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300319907
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG824250.061.179299
GAGCGTC1039700.048.616178
TCGGAAG1248900.041.1032872
AGAGCGT1262950.040.2615557
ATCGGAA1044050.039.2349851
CGGAAGA1394200.036.8623243
AAGAGCG1473450.034.797476
CGCCGTA525550.018.76531855-59
GAAGAGC3059700.017.2506565
TCGCCGT621600.015.85386655-59
GTCGCCG678350.014.78979455-59
GCCGTAT685050.014.64300155-59
CCGTATC747100.013.24185355-59
CAGTACG792450.012.80878430-34
CGTATCA806800.012.65362160-64
TGGTCGC806200.012.64911150-54
GCGTCGT809750.012.58574510-14
GTGGTCG904200.012.39747750-54
GGTCGCC805150.012.28381950-54
CGTCGTG840150.012.15887710-14
Produced by FastQC (version 0.11.5)