FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467646070
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG22749140.48646062608844337TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3819300.094.802341
TCGGAAG4001800.090.491522
CGGAAGA4214900.085.8198853
AGAGCAC6923050.052.4400757
AGCACAC7179100.050.606469
GAGCACA7335400.049.570658
GAAGAGC7455200.048.9147155
AAGAGCA10052500.036.497826
GGAAGAG11141500.033.0867544
TATGCCG3124850.024.93398545-49
CTCGTAT3152600.023.89937240-44
TCGTATC3281950.023.77954335-39
TCTCGTA3193250.023.61329840-44
CGTATGC3278150.023.5795545-49
GATCGGA1154150.023.5613611
TATCTCG3279700.022.80429840-44
ATGCCGT3417850.022.77527245-49
GTCACGA3447000.022.63959725-29
ATTCGTA3499950.022.15214335-39
GCCGTCT3487000.022.0814850-54
Produced by FastQC (version 0.11.5)