FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457489154
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC6633200.14499141546861677Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2301250.044.5571829
GAGCGTC2686700.038.2052088
ATCGGAA2871200.036.2032851
TCGGAAG3049700.034.1624262
AGAGCGT3026850.033.9944277
CGGAAGA3219850.032.3316043
AAGAGCG3504400.029.6908176
GAAGAGC6408650.016.6792645
CGCCGTA1421000.014.8223855-59
TCGCCGT1610350.013.11012855-59
GCCGTAT1674150.012.75946655-59
GTCGCCG1804100.011.781733555-59
CCGTATC1784100.011.70415355-59
CGTATCA1859250.011.53830260-64
TGGTCGC1921600.011.20546650-54
GGAAGAG10119500.010.9005554
GGTCGCC1976650.010.67115350-54
GTGGTCG2086950.010.38364950-54
GCGTCGT2241000.09.39718410-14
CGTCGTG2300200.09.19566410-14
Produced by FastQC (version 0.11.5)