FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480664373
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC9490210.1974394303611098Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2486050.058.924239
GAGCGTC2866950.051.2462438
ATCGGAA3082800.048.3846661
TCGGAAG3283600.045.3273472
AGAGCGT3266950.045.084917
CGGAAGA3457900.042.964753
AAGAGCG3761000.039.453566
GAAGAGC6804250.022.3233495
CGCCGTA1680950.017.9036455-59
TCGCCGT1857600.016.200355-59
GCCGTAT1944500.015.61051255-59
GTCGCCG2040000.014.79657555-59
GGAAGAG10581900.014.7358294
CCGTATC2049650.014.40436255-59
CGTATCA2120900.014.3194560-64
TGGTCGC2186200.014.00971750-54
GGTCGCC2227550.013.29797450-54
GTGGTCG2343000.013.12288850-54
GCGTCGT2428900.012.4560910-14
CGTCGTG2489350.012.17865710-14
Produced by FastQC (version 0.11.5)