FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485592853
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG23110340.4759201017318103TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4093800.096.3659741
TCGGAAG4267900.092.4569852
CGGAAGA4479750.087.985893
AGAGCAC7183750.054.972727
AGCACAC7484650.052.6425939
GAGCACA7647300.051.6774528
GAAGAGC7793850.050.8508455
AAGAGCA10424250.038.221776
GGAAGAG11657650.034.3707964
TATGCCG3224800.024.75787545-49
CTCGTAT3171400.023.75326340-44
CGTATGC3356550.023.42930645-49
TCTCGTA3235500.023.26833540-44
ATGCCGT3520250.022.6672145-49
CGCTCAT3505850.022.58856230-34
TATCTCG3296650.022.49544140-44
ACCGCTC3564800.022.22681630-34
TCGTATG3334900.022.20090940-44
TGCCGTC3534450.022.19999145-49
GCCGTCT3570750.021.8999350-54
Produced by FastQC (version 0.11.5)