FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485592853
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC25279960.520599919126075Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3939800.099.942759
GAGCGTC4332200.090.6927268
ATCGGAA4570750.087.048961
AGAGCGT4645950.084.738957
TCGGAAG4745700.083.6291662
CGGAAGA4931450.080.440693
AAGAGCG5078650.077.8490756
GAAGAGC8285700.048.1552665
GGAAGAG12380100.032.608884
CGCCGTA3219900.024.64313355-59
TCGCCGT3376250.023.4950755-59
GCCGTAT3478700.022.88024755-59
GTCGCCG3523700.022.4419555-59
TGGTCGC3667850.021.80717850-54
CCGTATC3618450.021.74729555-59
CGTATCA3689600.021.49501260-64
GGTCGCC3716550.021.2369750-54
CAGTACG3751950.020.99211530-34
GTGGTCG3826950.020.96196250-54
AGTACGT3877800.020.49178135-39
Produced by FastQC (version 0.11.5)