FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004972397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004972397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492730708
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG13132090.26651657359256775TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3030800.075.639281
TCGGAAG3179550.071.996422
CGGAAGA3399150.067.298963
AGAGCAC6165350.037.3807537
AGCACAC6450800.035.596299
GAGCACA6617500.034.8036588
GAAGAGC6775450.034.108925
AAGAGCA9486350.024.6474466
TATGCCG2064850.022.54091545-49
GGAAGAG10749450.021.8998224
CTCGTAT2033050.020.78780640-44
CGTATGC2224200.020.71801645-49
TCTCGTA2078150.020.2054940-44
ATGCCGT2391600.019.47765445-49
TGCCGTC2388300.019.109945-49
TCGTATG2207850.018.92066240-44
GCCGTCT2430050.018.79199850-54
ATCTCGT2350250.017.94023740-44
CCGGAGA2577550.017.82361830-34
ACTCCGG2622650.017.77196730-34
Produced by FastQC (version 0.11.5)