FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25056808
Sequences flagged as poor quality0
Sequence length35-71
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA1014150.062.8654217
GAGTCCT1852050.062.26849717
ATTCGAG523750.062.08211513
GGAGTCC1782500.062.0318716
TCGAGTC544900.061.95466615
GAGTCCG548850.061.87187617
AATCGAG341100.061.86395313
CGAGTCC822250.061.55009516
AGAGTCC523650.061.4499116
TGAGTCC423650.061.3665416
AAAGGAG885650.061.01074613
ATTGGAG951150.060.93384613
ATAGGAG419900.060.7361713
AGTCCGA279250.060.6636918
AATGGAG831250.060.5989313
AGGAGTC684900.060.43955215
AGTCCAT330300.060.31553318
AGTCCAG666100.060.1804818
TTCGAGT358200.060.0827314
AAGGAGT509800.060.0086114
Produced by FastQC (version 0.11.5)