FastQCFastQC Report
Mon 23 Jan 2023
EGAF00004973392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384993963
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA217128215.639782200948434TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA20669440.5368769899386708TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA19491260.5062744321525894TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4990940.12963683796776834TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4988070.12956229134429312TruSeq Adapter, Index 25 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4796430.12458455095307559TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4647810.12072423068098861TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4543840.11802366885425682TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4263040.11073004799298633TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4166520.10822299569409093TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4161900.10810299381239909TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4078300.10593153119130858TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4072150.10577178842671878TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4037990.10488450178633062TruSeq Adapter, Index 25 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)