FastQCFastQC Report
Mon 19 Dec 2022
EGAF00004973394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379708782
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA78126192.0575291829831843TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA65705641.7304219210816145TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15079950.3971451468825917TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA13637540.3591578769437047TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6063680.1596929090778838TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA4972710.13096115327667085TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA4272210.11251280461561725TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)