FastQCFastQC Report
Fri 30 Dec 2022
EGAF00004973398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386791415
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA95234422.462164782018236TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA55740801.4411074764934997TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14647450.3786911868248162TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA10598550.27401202790398027TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6021690.15568313479760143TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA3940770.10188359532230051TruSeq Adapter, Index 25 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)