FastQCFastQC Report
Thu 19 Jan 2023
EGAF00004973402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384993832
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA63796901.6570888855175219TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA53062731.3782748083091367TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12592140.32707381140589287TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA12064060.3133572280191751TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8003120.2078765770979936TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCCCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA6717290.1744778602063422TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAAA4608030.11969100845231204TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)