FastQCFastQC Report
Mon 30 May 2022
EGAF00004973403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384993832
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGGGG16601980.43122716833551766Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGAGGGGGG5941800.1543349400984689Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGGGGG5239270.13608711528656386Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGG4517010.11732681473193057Illumina Single End PCR Primer 1 (100% over 56bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)