FastQCFastQC Report
Fri 27 May 2022
EGAF00004973404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395990777
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA236220045.965291459300831TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18648720.47093824106918525TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11518410.2908757140068442TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5419670.13686354114252514TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5362330.13541552761972536TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5055710.12767241798664417TruSeq Adapter, Index 25 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5028980.1269974022652553TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4930040.12449885922469352TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4595980.11606280415970395TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4578590.11562365251754335TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4571690.11544940603502994TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4445970.1122745846174089TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4377490.11054525141124688TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4145190.10467895316662894TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4104320.10364685842165461TruSeq Adapter, Index 25 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)