FastQCFastQC Report
Mon 19 Dec 2022
EGAF00004973406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401176213
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA255748076.3749559847408TruSeq Adapter, Index 25 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18187690.4533591327360179TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10910810.2719705118708023TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5854870.14594260103851173TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5788250.1442819841364822TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5452030.13590112831540188TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5350180.13336234369409136TruSeq Adapter, Index 25 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5253010.13094021603917977TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4972780.12395500627550915TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4963410.12372144307568904TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4808620.11986303883874591TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4783030.11922516452888497TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4734370.11801223119876252TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4436590.11058955781109583TruSeq Adapter, Index 25 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4079490.10168823244762022TruSeq Adapter, Index 25 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)