FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456214796
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG16407300.3596398044047655TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4288700.071.8533251
TCGGAAG4427200.069.414122
CGGAAGA4602950.066.7196963
AGAGCAC7073550.043.609637
AGCACAC7352350.042.02239
GAGCACA7471350.041.3292358
GAAGAGC7655350.040.4647835
AAGAGCA10123450.030.7868676
GGAAGAG11296350.027.7468664
TATGCCG2832500.021.84298545-49
CGTATGC2963450.020.7120945-49
CTCGTAT2617900.020.2301740-44
ATGCCGT3100150.019.88149545-49
TCTCGTA2664400.019.7912140-44
TGCCGTC3111650.019.5375745-49
GCCGTCT3130050.019.36765950-54
TTACTCG3146200.019.2854730-34
TACTCGA3172800.019.16676135-39
TCGTATG2762650.018.92692640-44
ATCTCGT2908350.018.13960640-44
Produced by FastQC (version 0.11.5)