FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456214796
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC19532050.4281327605166054Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4061500.075.556299
GAGCGTC4491850.068.2868048
AGAGCGT4750000.064.738067
ATCGGAA4876200.064.235241
TCGGAAG4999900.062.3024442
CGGAAGA5173050.060.078823
AAGAGCG5264450.058.805926
GAAGAGC8248350.038.038715
GGAAGAG11938250.026.6264534
CGCCGTA2758350.022.24774755-59
TCGCCGT2901050.021.06593955-59
GCCGTAT2989900.020.57619355-59
GTCGCCG3098850.019.7872755-59
CCGTATC3109750.019.5603255-59
TGGTCGC3194000.019.3313650-54
CGTATCA3163750.019.28705460-64
GGTCGCC3278350.018.72517850-54
GTGGTCG3336600.018.54645750-54
TCTCGGT3785650.016.57130845-49
TCTTCGC3762150.016.39846630-34
Produced by FastQC (version 0.11.5)