FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478238665
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG15150730.31680269933841504TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3870150.070.357141
TCGGAAG3980950.068.336822
CGGAAGA4256500.063.884763
AGAGCAC6703950.040.9567387
AGCACAC7004850.039.17099
GAGCACA7145650.038.459938
GAAGAGC7418200.036.9628525
AAGAGCA9949900.027.7833986
GGAAGAG11128650.025.051934
TATGCCG2619950.021.1835845-49
CGTATGC2720950.020.2214345-49
CTCGTAT2442250.020.01150140-44
TCTCGTA2492750.019.5752740-44
CGATAGA2823000.019.315735-39
AGCGATA2908900.019.02997830-34
ATGCCGT2935750.018.90485445-49
GCGATAG2887700.018.77508730-34
GCCGTCT2952950.018.48269750-54
TCGTATG2622700.018.33614740-44
TGCCGTC2985300.018.2729845-49
Produced by FastQC (version 0.11.5)