FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478238665
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC17572230.36743641378306374Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3712450.073.753559
GAGCGTC4110750.066.6958548
AGAGCGT4414800.062.2189457
ATCGGAA4468600.062.0424041
TCGGAAG4592050.060.284622
CGGAAGA4856700.056.9756623
AAGAGCG4943500.055.8755956
GAAGAGC8058250.034.718895
GGAAGAG11800200.024.0550384
CGCCGTA2634800.021.00998555-59
TCGCCGT2831100.019.59111255-59
GCCGTAT2862350.019.40153755-59
GTCGCCG2979750.018.60696455-59
TGGTCGC3058900.018.316650-54
CCGTATC2996950.018.21522955-59
CGTATCA3033600.018.19129460-64
GTGGTCG3181150.017.65600450-54
GGTCGCC3139650.017.55687350-54
CCGTGTA3502800.016.14871635-39
TCTCGGT3600350.015.7716945-49
Produced by FastQC (version 0.11.5)