FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477438769
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG10846020.22717091078960955TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4422200.040.4725841
TCGGAAG4530050.039.3915142
CGGAAGA4742200.037.664453
AGAGCAC7131550.025.3372277
AGCACAC7345700.024.5860759
GAGCACA7557400.023.934788
GAAGAGC7873600.023.0855885
AAGAGCA10284850.017.8613936
TATGCCG2232200.016.34618845-49
GGAAGAG11535950.016.0528264
CGTATGC2386650.015.13164745-49
CTCGTAT2338850.014.83283840-44
TCTCGTA2429650.014.27254440-44
ATGCCGT2603250.014.12322745-49
TCGTATG2486000.013.724524540-44
TATCTCG2529400.013.53660540-44
GCCGTCT2693200.013.44687150-54
TGCCGTC2693650.013.42590345-49
ATCTCGT2753050.012.67072540-44
CCGTCTT2913000.012.64375650-54
Produced by FastQC (version 0.11.5)