FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477438769
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC11737320.24583927326605517Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4297600.042.1198359
GAGCGTC4763550.038.0325058
AGAGCGT4998350.036.316957
ATCGGAA5092100.036.0003621
TCGGAAG5217550.035.0439452
CGGAAGA5436250.033.6469883
AAGAGCG5526700.033.0273446
GAAGAGC8605250.021.6159845
CGCCGTA2256300.016.30393255-59
GGAAGAG12267100.015.4635264
GCCGTAT2403100.015.34537655-59
CGTATCA2556950.014.35750660-64
CCGTATC2537600.014.31429555-59
TCGCCGT2693250.013.80794855-59
TGGTCGC2821150.013.14398350-54
GTGGTCG3041500.012.28229750-54
GGTCGCC3142650.011.73335550-54
GTCGCCG3207900.011.66232955-59
GTACGTC3157700.011.56895430-34
TACGTCC3211850.011.47776330-34
Produced by FastQC (version 0.11.5)