FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459765713
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG16455250.35790511416409165TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2870000.098.447571
TCGGAAG3043950.092.77982
CGGAAGA3267500.086.411493
AGAGCAC5910850.047.941147
AGCACAC6243650.045.4020279
GAGCACA6371750.044.5188268
GAAGAGC6471500.043.9771465
AAGAGCA9047250.031.6988076
GGAAGAG10145600.028.3967174
TCCGCGA2231850.025.44619830-34
CGCGAAA2217850.025.35503835-39
CCGCGAA2267800.024.68093130-34
TATGCCG2389450.023.94355645-49
CTCGTAT2312150.022.50342640-44
CGTATGC2529350.022.35324145-49
CTCCGCG2559100.022.30043430-34
GCGAAAT2556050.022.26684435-39
TCTCGTA2358350.022.03430440-44
ACTCCGC2672850.021.37796230-34
ATGCCGT2683850.021.2922645-49
Produced by FastQC (version 0.11.5)