FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459765713
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC18271420.39740718986585244Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3468500.081.8014459
GAGCGTC3852550.073.671348
ATCGGAA4073950.071.052321
TCGGAAG4259350.067.7521062
AGAGCGT4261550.066.815397
CGGAAGA4482250.064.275213
AAGAGCG4805100.059.774646
GAAGAGC7701000.037.7159045
GGAAGAG11592750.025.4194364
CGCCGTA2763650.020.73901455-59
TCGCCGT2908250.019.67345655-59
GCCGTAT3008150.019.15988555-59
GTCGCCG3111550.018.46912855-59
CCGTATC3098550.018.30001355-59
CGTATCA3171300.018.03735460-64
TGGTCGC3275500.017.74241450-54
GGTCGCC3308550.017.31451850-54
GCGTCGT3356950.017.08563810-14
GTGGTCG3463650.016.85899450-54
CGTCGTG3426700.016.78229110-14
Produced by FastQC (version 0.11.5)