FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186857709
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG8394230.44923113126684006TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3148200.044.1031881
TCGGAAG3158650.043.927212
CGGAAGA3188150.043.4712033
AGAGCAC4039550.034.399137
AGCACAC4077750.033.9701429
GAGCACA4186050.033.195178
GAAGAGC4372400.031.9363165
AAGAGCA5312900.026.4097676
GGAAGAG5864050.024.0837654
TATGCCG1502200.018.72973445-49
CGTATGC1588850.017.52032745-49
ATGCCGT1613400.017.49993145-49
CTCGTAT1521800.017.4175940-44
GCCGTCT1622250.017.12286850-54
TGCCGTC1625600.017.0778745-49
TCTCGTA1554500.017.0260240-44
CGTCTTC1711550.016.5191650-54
GCGCATC1700850.016.50593235-39
TCGTATG1599550.016.3190140-44
CCGTCTT1766300.016.01614250-54
Produced by FastQC (version 0.11.5)