FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478758221
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1409600.033.0422481
TCGGAAG1576400.029.688292
CGGAAGA1806950.025.91453
TATGCCG812050.012.17107545-49
AGAGCAC4529400.010.7924377
CTCGTAT876550.010.48827940-44
CGTATGC947100.010.41872245-49
AGCACAC4867450.010.1948069
GAGCACA4986150.09.9739178
GAAGAGC5073900.09.8417145
TCTCGTA939100.09.82983440-44
TATCTCG993750.09.2965540-44
ATGCCGT1104700.09.15415445-49
TGCCGTC1139900.08.58911445-49
GCCGTCT1177250.08.49955550-54
TCGTATG1060350.08.4555840-44
ATCTCGT1182400.07.954312340-44
ACACGTC1420550.07.177369610-14
CGTCTGA1436350.07.11437715-19
CGTCTTC1501750.06.893683450-54
Produced by FastQC (version 0.11.5)