FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478758221
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1809000.025.8019229
GAGCGTC2208500.021.3004428
ATCGGAA2402150.019.8422971
TCGGAAG2587950.018.4917962
AGAGCGT2575450.018.3516397
CGGAAGA2812500.017.0357363
AAGAGCG3034750.015.7819116
CGCCGTA1011650.09.60615855-59
GAAGAGC6098100.08.3937445
TCGCCGT1177150.08.32702655-59
GCCGTAT1283100.07.79762555-59
GTCGCCG1356650.07.335340555-59
CCGTATC1398550.06.947638555-59
TGGTCGC1463700.06.870192550-54
CGTATCA1472650.06.859985460-64
GGTCGCC1534000.06.410742850-54
GTGGTCG1616850.06.31090250-54
CAGTACG1677650.05.736814530-34
GCGTCGT1733500.05.63531310-14
AGTACGT1806700.05.58375935-39
Produced by FastQC (version 0.11.5)