FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453819786
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG14634280.32246897229818006TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2599150.097.250421
TCGGAAG2769650.091.167982
CGGAAGA2998000.084.197373
AGAGCAC5569250.045.5698937
AGCACAC5900350.042.9427079
GAGCACA6013550.042.283798
GAAGAGC6123600.041.625685
AAGAGCA8654850.029.680196
GGAAGAG9608300.026.898214
TATGCCG2164900.023.94797745-49
TCGTATC2314600.022.43635435-39
CTCGTAT2120400.022.34265940-44
CGTATGC2299500.022.34258345-49
TCTCGTA2152100.021.99267440-44
GTCACGA2402900.021.57362225-29
ATGCCGT2443950.021.19877245-49
TATCTCG2212750.021.1553540-44
GCCGTCT2477700.020.62396650-54
ATTCGTA2505200.020.6130535-39
TGCCGTC2454800.020.48859245-49
Produced by FastQC (version 0.11.5)