FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453819786
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC16356310.36041421076338875Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2648200.095.903579
GAGCGTC2998700.084.803348
ATCGGAA3153800.082.026131
TCGGAAG3325100.077.5340962
AGAGCGT3337700.076.379077
CGGAAGA3549200.072.533923
AAGAGCG3774450.068.056636
GAAGAGC6682900.038.9075365
GGAAGAG10194450.025.88214
CGCCGTA2168850.023.8429155-59
TCGCCGT2302750.022.46657255-59
GCCGTAT2416350.021.51894855-59
GTCGCCG2452100.021.1780255-59
TGGTCGC2574350.020.34125750-54
GCGTCGT2561550.020.32017510-14
CCGTATC2522800.020.02305455-59
CGTCGTG2622450.019.88588110-14
CGTATCA2612000.019.86153260-64
GGTCGCC2613700.019.535850-54
GTGGTCG2701300.019.5216250-54
Produced by FastQC (version 0.11.5)