FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253561091
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC7706140.3039165027097947Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2087950.058.4431769
GAGCGTC2304150.053.0380638
AGAGCGT2516100.048.738957
ATCGGAA2567150.048.2952651
TCGGAAG2608700.047.2909432
CGGAAGA2747450.044.8702283
AAGAGCG2823350.043.5903476
GAAGAGC4390750.028.4818655
GGAAGAG6371250.019.904734
CGCCGTA1282000.019.11089755-59
TCGCCGT1366650.017.90065255-59
GCCGTAT1392600.017.63683555-59
GTCGCCG1431600.017.03991355-59
CCGTATC1465500.016.5893655-59
CGTATCA1494800.016.31805260-64
TGGTCGC1513300.016.29481350-54
GGTCGCC1534700.015.98254950-54
GTGGTCG1575650.015.68130850-54
TCTCGGT1815950.013.81963245-49
TCGGTGG1854550.013.39911245-49
Produced by FastQC (version 0.11.5)