FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393715428
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG23145510.5878740926555716TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6139850.064.931211
TCGGAAG6202800.064.2516862
CGGAAGA6265200.063.4878433
AGAGCAC8232200.048.4116487
AGCACAC8337950.047.7499479
GAGCACA8521450.046.7786988
GAAGAGC8835750.045.2073145
AAGAGCA10936950.036.6838386
GGAAGAG11954250.033.753694
TATGCCG3631750.022.16549545-49
CGTATGC3786400.021.08939745-49
ATGCCGT3871300.020.80254245-49
CTCGTAT3599600.020.64062940-44
TCTCGTA3636450.020.37764540-44
GCCGTCT3900250.020.36830950-54
TGCCGTC3887950.020.22643345-49
TCGTATC4052650.019.99638635-39
CGTCTTC4106900.019.6639850-54
TATCTCG3762000.019.60354440-44
TCGTATG3696300.019.5460240-44
Produced by FastQC (version 0.11.5)