FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393715428
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC24522790.6228557037902005Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5534500.071.638259
GAGCGTC5912950.067.054048
AGAGCGT6266550.063.4064647
ATCGGAA6572700.061.1782231
TCGGAAG6611700.060.6818472
CGGAAGA6693550.059.9191323
AAGAGCG6728250.059.386096
GAAGAGC9224050.043.6435135
GGAAGAG12461200.032.6636734
CGCCGTA3509950.022.88318655-59
TCGCCGT3648700.021.9355-59
GCCGTAT3714950.021.67738755-59
GTCGCCG3813400.021.07024655-59
CGTATCA3868000.020.6288560-64
CCGTATC3820650.020.57931955-59
TGGTCGC3970300.020.38964850-54
GGTCGCC3996300.019.8867850-54
GTGGTCG4131550.019.61525750-54
TCTCGGT4522000.018.10705445-49
CTCGGTG4670200.017.55045745-49
Produced by FastQC (version 0.11.5)