FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457224611
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3859350.016.0888449
GAGCGTC4317450.014.45229158
AGAGCGT4704500.013.3541857
ATCGGAA5091600.012.4721011
TCGGAAG5137150.012.319262
CGGAAGA5229050.012.1517123
AAGAGCG5215350.012.118626
CGCCGTA1292200.09.79217555-59
TCGCCGT1472550.08.661798555-59
GCCGTAT1524750.08.47650655-59
GAAGAGC8234000.08.0727935
GTCGCCG1644600.07.85966455-59
CCGTATC1641450.07.784658455-59
CGTATCA1689400.07.740669360-64
TGGTCGC1836600.07.12822950-54
GGTCGCC1854150.06.95832750-54
GTGGTCG1980200.06.65450250-54
GCGTCGG482000.06.4411345145
GGAAGAG12015450.05.8333314
TCTCGGT2486700.05.48373945-49
Produced by FastQC (version 0.11.5)