FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447986450
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG5509160.12297604090480861TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3107250.032.4140431
TCGGAAG3210450.031.3963342
CGGAAGA3364900.030.0104063
AGAGCAC5696100.018.1117327
AGCACAC5954150.017.3474759
GAGCACA6110700.016.978978
GAAGAGC6313600.016.474925
TATGCCG1311400.015.556272545-49
CGTATGC1468100.013.85930245-49
CTCGTAT1345250.013.19504840-44
ATGCCGT1582000.012.97329845-49
TGCCGTC1604150.012.59890245-49
TCTCGTA1419100.012.51859540-44
GCCGTCT1642650.012.31338950-54
AAGAGCA8738050.012.1593216
TCGTATG1530600.011.48633940-44
GTCACGA1884900.010.93450125-29
CGTCTTC1908700.010.89784650-54
GGAAGAG9923950.010.8276064
ATCTCGT1674100.010.69057240-44
Produced by FastQC (version 0.11.5)