FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447986450
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC6484060.14473785981696544Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2644700.037.883649
GAGCGTC3056950.032.8509068
AGAGCGT3373250.029.9110627
ATCGGAA3530150.028.724691
TCGGAAG3630050.027.9002592
CGGAAGA3762700.026.983833
AAGAGCG3813100.026.5546556
CGCCGTA1215700.016.66407455-59
GAAGAGC6751000.015.5097915
TCGCCGT1367100.014.86845355-59
GCCGTAT1459550.014.03991355-59
GTCGCCG1500800.013.60378455-59
CCGTATC1587700.012.79253955-59
CGTATCA1650100.012.45211160-64
TGGTCGC1660250.012.44113950-54
GGTCGCC1693850.012.00945950-54
GTGGTCG1798050.011.55540450-54
GGAAGAG10397300.010.3774824
TCTCGGT2224500.09.55406945-49
TCGGTGG2231950.09.39481745-49
Produced by FastQC (version 0.11.5)