FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464062798
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC16331160.35191702654001583Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3474200.072.4952859
GAGCGTC3894950.064.70688
AGAGCGT4167900.060.6190647
ATCGGAA4230200.060.440091
TCGGAAG4357550.058.4962042
CGGAAGA4527000.056.2461743
AAGAGCG4649100.054.6845446
GAAGAGC7671300.033.6366045
GGAAGAG11331000.023.100374
CGCCGTA2315700.021.84146955-59
TCGCCGT2475850.020.4192955-59
GCCGTAT2554550.019.88722255-59
GTCGCCG2630100.019.2542555-59
TGGTCGC2734800.018.7131850-54
CCGTATC2691750.018.67274955-59
CGTATCA2747000.018.36085960-64
GGTCGCC2806800.018.04572950-54
GTGGTCG2868000.017.91380750-54
TCTCGGT3288850.015.77698145-49
TCGGTGG3294450.015.59266345-49
Produced by FastQC (version 0.11.5)