FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470622617
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT11138520.23667625816631757Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2838950.063.4270061
GAGCGTC3086600.057.9235539
ATCGGAA3333950.054.087912
AGAGCGT3433900.052.1302268
TCGGAAG3470400.051.7517933
CGGAAGA3711150.048.4319044
AAGAGCG3867050.046.394667
GAAGAGC6977750.026.2278736
CGCCGTA2011450.022.13682755-59
TCGCCGT2154750.020.74400155-59
GCCGTAT2267400.019.66736855-59
GTCGCCG2298000.019.46034655-59
TGGTCGC2410450.018.60771850-54
CGTATCA2471400.018.05538660-64
GTGGTCG2535950.017.78463650-54
GGAAGAG10774350.017.2942645
GCGTCGT2629550.016.96618310-14
AGTACGT2666450.016.7378235-39
CGTCGTG2677750.016.67920110-14
AGCGTCG2699350.016.258989
Produced by FastQC (version 0.11.5)