FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438316898
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG25067830.571911101634051TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5598250.085.082411
TCGGAAG5736750.082.746142
CGGAAGA5881500.080.464193
AGAGCAC8179700.057.787997
AGCACAC8395750.056.2822349
GAGCACA8544750.055.3395238
GAAGAGC8764700.054.1037525
AAGAGCA11179950.042.5639466
GGAAGAG12222850.039.122114
TATGCCG3920950.024.3142545-49
CGCGAAA3926300.023.91618335-39
TCCGCGA3978350.023.89564130-34
CCGCGAA4005800.023.49845730-34
CGTATGC4072050.023.08199145-49
CTCGTAT3532750.022.87774740-44
ATGCCGT4175950.022.64066545-49
GCGAAAT4204650.022.43220735-39
TCTCGTA3587150.022.41884240-44
TGCCGTC4184700.022.31304745-49
GCCGTCT4178850.022.13753350-54
Produced by FastQC (version 0.11.5)